Purpose: Del Nido cardioplegia (DNC) has been shown to be safe in adult cardiopulmonary bypass surgery but its effect on Isolated Coronary Artery Bypass Grafting (Iso-CABG) surgeries is still being reviewed in literatures. Herein, we present the clinical outcome of Del Nido versus Blood Cardioplegia (BC) in patients undergoing Iso-CABG. Methodology: This study included 62 patients who underwent Iso-CABGs with cardiopulmonary bypass (CPB) from January to May 2024 by a single surgeon at Liverpool hospital, Australia; out of which 34 patients received DNC and the remaining 28 patients, BC. Preoperative characteristics, intraoperative data, and postoperative outcomes were compared. Results: Cardiovascular comorbidities and Pre-operative rate of presentation with acute coronary syndrome (ACS) were similar between both groups (P>0.05). The aortic Cross Clamp Time (CCT) as well as Cardiopulmonary Bypass (CPB) did not show significant difference between the DNC and BC groups (71.1±18.7 vs. 76.6±22.4; P=0.142) with similar number of distal anastomosis between groups (P=0.122). DNC group required similar ventilation time, ICU stay and total hospital stay compared to BC group with similar post-operative in-hospital Major Adverse Cardiovasular Events (MACE) (P>0.05). Moreover, there was a significant higher post-operative Troponin levels in the first 24hrs within BC group compared to the DNC (P=0.0001). Conclusions: The utilization of DNC is associated with comparative operative time and post-operative hospitalization with BC. DNC is associated with better cardiac protection as reflected by lower post-operative troponin leak. Further evaluation for the superiority of DNC over BC needs larger cohort studies.